Human LATS1 (Serine/threonine-protein kinase LATS1) ELISA Kit

Human LATS1 (Serine/threonine-protein kinase LATS1) ELISA KitThe Human LATS1 (Serine threonine protein kinase LATS1) ELISA Kit measures LATS1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LATS1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human LATS1 (Serine/threonine-protein kinase LATS1) ELISA Kit measures LATS1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LATS1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with LATS1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of LATS1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.