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Description
Ubiquitin Recombinant Rabbit mAb (SDT-R095)Product Specification Host Rabbit Antigen Ubiquitin Synonyms N A Immunogen N A Location Cytoplasm, Nucleus Accession P62987 Clone Number SDT R095 Antibody Type Recombinant mAb Application WB, IHC P, ICC, ICFCM Reactivity Species Independent Purification Protein A Concentration 0. 5 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as
Product Specification
| Host | Rabbit |
| Antigen | Ubiquitin |
| Synonyms | N/A |
| Immunogen | N/A |
| Location | Cytoplasm, Nucleus |
| Accession | P62987 |
| Clone Number | SDT-R095 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:2000 | |
| ICFCM | 1:500 | |
| ICC | 1:500 |
Background
Protein ubiquitination is a dynamic multifaceted post-translational modification involved in nearly all aspects of eukaryotic biology. Once attached to a substrate, the 76-amino acid protein ubiquitin is subjected to further modifications, creating a multitude of distinct signals with distinct cellular outcomes, referred to as the 'ubiquitin code'. Ubiquitin can be ubiquitinated on seven lysine (Lys) residues or on the N-terminus, leading to polyubiquitin chains that can encompass complex topologies. Alternatively or in addition, ubiquitin Lys residues can be modified by ubiquitin-like molecules (such as SUMO or NEDD8). Finally, ubiquitin can also be acetylated on Lys, or phosphorylated on Ser, Thr or Tyr residues, and each modification has the potential to dramatically alter the signaling outcome [PMID: 27012465].
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